standardization of elisa for detection of avian influenza virus antibodies

Standardization of ELISA for Detection of Avian Influenza Virus Antibodies

Standardization of ELISA for Detection of A vian Influenza Virus

An indirect enzyme-Iinked immunosorbent assay (ELISA) was developed for the rapid and efficient large scale screening of antibodies to avian influenza virus (AIV) infection in chicken. Antigen was a whole-purified influenza virus produced from the H9N2 subtype. Optimum dilution for goat anti-chicken conjugate to be used in the ELISA was 1: 1000, as determined by signal-to-noise ratio. The antig...

Development of blocking ELISA for detection of antibodies against avian influenza virus of the H7 subtype.

BACKGROUND AND PURPOSE The conventional method used for subtyping of antibodies against avian influenza viruses is hemagglutination inhibition (HI) test. However, the HI test is laborious and requires preparation of antigen from viable viruses that might be hazardous. The aim of this study was to develop a blocking enzyme-linked immunosorbent assay (B-ELISA) for detection of antibody of avian i...

Development of a Nano-ELISA system for the rapid and sensitive detection of H9N2 avian influenza

Influenza is one of the most important viral diseases that is common among the birds and the mammalsand is caused by specific viruses that belong to the Orthomyxoviridae family. Migratory aquatic birds arethe reservoir of the disease and there is a likelihood of the disease in any region. There are differentmethods for detecting the avian influenza, but by the point of detection rates, the ELIS...

Production of monoclonal antibodies against recombinant nucleoprotein of avian influenza virus, serotype H9N2

Background: Avian influenza viruses (AIVs) including the subtype H9N2 cause considerable financial losses to poultry industries. Rapid and accurate diagnosis of avian influenza (AI) infection is important in control and eradication programs. OBJECTIVES: The aim of this study was to produce monoclonal antibodies (MAbs) specific for the nucleocapsid protein )NP (of AIV H9N2 subtype to improve dia...

A Reverse transcription-PCR assay for detection of type A influenza virus and differentiation of avian H7 subtype

Abstract : Avian influenza virus (AIV) infection is a major cause of influenza mortality in birds and can cause human mortality and morbidity. Although the risk of infection with avian influenza virus (AIV) is generally low for most people, the pathogenic virus can cross the species barrier and acquires the ability to infect and be transmitted among the human population; therefore the ra...